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  eBlot蛋白转印系统-10分钟轻松完成。提供样机  
 


产品名称:  eBlot蛋白转印系统-10分钟轻松完成。提供样机
产品编号:  N201367182518
发布日期:  2014-9-20
查?#21019;?#25968;:  293 
  产 品 详 细 介 绍



eBlotTM 蛋白转印系统
是一个高效的蛋白快速转印系统,可以实现在7-10分钟的时间内稳定、高效、快速、可?#24247;?#23545;各种类型的凝胶进行蛋白质印迹(Western Blot)实验。eBlotTM 蛋白转印系统包括eBlotTM 蛋白转印仪、eBlotTM蛋白转印试剂盒两部分。配合试剂盒内含有特定缓冲液的正负极滤纸,eBlotTM蛋白转印仪可在仪器的正极和负极之间形成一个匀?#24247;?#22330;。在电场驱动下,凝胶中带有负电荷的蛋白质分子发生定向移动,从而快速的转移到NC或PVDF膜上,完成转印过程。
与传统的湿式转印、半干式系统相比eBlotTM 蛋白转印系统具有显著的优点:
1. 快速高效,可在7-10分钟内完成转印过程
2. 操作简便,人性化的流程设?#21697;?#20415;实验人员操作,减少人为操作失误
3. 不含甲醇等有害成分
4. 适用性强,可用于不同类型的聚丙烯酰胺凝胶
5. 检测灵敏度高,优于传统湿法转印

 

 
The eBlot ® Protein Transfer System is based on the proprietary fast semi-dry blotting technique developed by GenScript. To use the eBlot ® Protein Transfer System for rapid protein blotting, assemble the eBlot ® Protein Transfer Pad with your pre-run gel and the membrane on the eBlot ® Protein Transfer Device. A certain definite voltage between electrodes allows for rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane within 7-10 minutes.
protein gel staining Figure 1. System Mechanism
The eBlot ® Cathode Pad andeBlot ® Anode Pad act as ion reservoirs that contain the appropriate anode and cathode buffers. A certain definite voltage between electrodes drive a rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane.

  Shortened workflow increases your productivity  
   
The eBlot ® Protein Transfer System is faster and provides superior transfer quality compared to the conventional semi-dry western transfer and wet transfer systems (Table 1, Figure 2 and 3).
Table 1. Fast protein transfer with the eBlot ®
eblot system

  High and consistent protein transfer efficiency  
   
In addition to ease and convenience, the eBlot ® Protein Staining System provides higher detection sensitivity comparable to that of traditional protein transfer methods (Figure 3).
eblot transferred      Figure 2. Higher transfer quality of eBlot ® Protein Transfer System
(A) Comparison of protein transfer between eBlot® Protein Transfer System and a semi-dry western transfer method. (B) Comparison of protein transfer between eBlot ® Protein Transfer System and a tank wet western transfer method. Proteins were separated with Genscript Express™ PAGE Gels 8-16% and transferred to nitrocellulose . (A) Lanes 1-5 and 6-10: 0.313 µl, 0.625 µl, 1.25 µl, 2.5 µl, and 5.0 µl Genscript EasyWestern Protein Standard (22, 40, 60, 85, and 120 kDa); (B) Lanes 1-5 and 6-10: 5.0 µl, 2.5 µl, 1.25 µl, 0.625 µl, and 0.313 µl Genscript High Range EasyWestern Protein Standard (60, 85, 120, 160 and 200 kDa).
  Optimized self-contained system offers easy and convenient procedures  
   
The eBlot ® Protein Transfer System is created as a self-contained system. All you need to do is to simply assemble the eBlot ® Protein Transfer Pads and membrane together with the pre-run gel, then press the run button (Figure 3). 

eblot Protein transfer System
Figure3. eBlot ® Protein Transfer System



  Suitable for various types of mini gels  
   
The eBlot ® Protein Transfer System is designed to work with multiple types of mini polyacrylamide gels, including Tris-Glycine, Bis-Tris, Tris-Tricine, and Tris-Acetate gels, etc.

       protein gel staining
       protein gel staining
Available Video
eBlot™ Protein Transfer System
 Watch the video and see the  eBlot ® System in action
eBlot ® Resources
 eBlot ® User Manual
 eBlot ®Quick Reference Card 
 eBlot ® FAQ
 eBlot ® Flyer 
 eBlot ® Troubleshooting
Promotion
 Buy 1 get 6, Free consumbles for 4 months
 Free Trial Program 

 

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最新2018十二生肖表图