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您的位置:代理品牌>> Epicentre
Epicentre Biotechnologies是美国分子生物学产品专业生产商,其在基因组研究、建库,蛋白研究和RNA分析方面都有自己的特色产品。CopyControl文库系列产品闻名于世。转座子系统也是其特色产品。另外还有SNP突变检测、测序、DNA指纹图谱分析方面的产品,涉及面广。
 
 

基因文库构建相关产品Genomic Library Production

Genomic Library Production

A Brief Guide to Genomic Library Production 基因文库产品选择指南

Fosmid Cloning Kits

·         CopyControl™ Fosmid Library Production Kit
CopyControl™ HTP Fosmid Library Production Kit

·         EpiFOS™ Fosmid Library Production Kit

·         CopyControl™ BAC Autoinduction Solution
CopyControl™ Fosmid Autoinduction Solution

Fosmid Clone Purification

·         FosmidMAX™ DNA Purification Kit

Bacterial Artificial Chromosome (BAC) Cloning Kits

·         CopyControl™ BAC Cloning Kits (BamH I, EcoR I, or Hind III)
CopyControl™ pCC1BAC™ Cloning-Ready Vectors (BamH I, EcoR I, or Hind III)

·         pIndigoBAC-5 (BamH I Cloning-Ready) Vector 
pIndigoBAC-5 (Hind III Cloning-Ready) Vector

Converting Single-Copy BAC or Fosmid Clones to Multi-Copy-Inducible 转换单拷贝至诱导多拷贝中

·         EZ-Tn5™ <oriV/KAN-2> Insertion Kit 转座插入试剂盒

BAC Clone Purification

·         BACMAX™ DNA Purification Kit

·         BACMAX96™ DNA Purification Kit

Sizing BAC Clones 筛查BAC克隆

·         BAC-Tracker™ Supercoiled DNA Ladder   超螺旋DNA分子量标准

Cosmid Cloning Kits

·         pWEB-TNC™ Cosmid Cloning Kit 
pWEB™ Cosmid Cloning Kit

Phage T1-Resistant Competent Cells 感受态细胞

·         Phage T1-resistant TransforMax™ EPI300™-T1R Electrocompetent E. coli and TransforMax™ EPI300™-T1R Chemically Competent E. coli

·         Phage T1-resistant TransforMax™ EC100™-T1R Electrocompetent E. coli

High-Efficiency Lambda Packaging Extracts 高效Lambda包装试剂盒

·         MaxPlax™ Lambda Packaging Extracts

 

Epicentre产品Competent Cells 试剂盒

TransforMax EPI300 Electrocompetent E.coli

Applications

·         Generation of inducible copy-number clones using the CopyControl™ Cloning System.

 

TransforMax™ EPI300™ E. coli cells lack the tonA gene and are engineered for use with EPICENTRE's CopyControl™ cDNA, Gene, and PCR Cloning Kit and other CopyControl Cloning Systems* that do not require phage T1-resistant cells. The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV origin of replication, such as that in CopyControl pCC1™ vectors or in clones that are retrofitted with CopyControl capability using theEZ-Tn5™<oriV/KAN-2> Insertion Kit. On LB chloramphenicol plates or in LB or SOC media supplemented with chloramphenicol, CopyControl clones grown in TransforMax EPI300 E. coli replicate at single-copy number from the F-factor replicon because expression of the trfA gene is repressed. Addition of CopyControl Induction Solution induces the cells to express the trfA gene product and induces their replication at high-copy number from oriV (Fig. 1). Replication from oriV results in higher yields and higher purity of cloned DNA.

Benefits

·         Copy-number of clones is under tight control of an inducible promoter linked to thetrfA gene.

·         High transformation efficiency with clones of all sizes.

·         lacZΔM15 for blue/white screening of recombinants.

·         Restriction-minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] phenotype enables efficient cloning of methylated DNA.

·         Endonuclease-minus (endA1), to ensure high yields of DNA.

·         Recombination-minus (recA1), for greater stability of large insert clones.

 Figure 1. Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™ E. coli. The yield of BAC DNA from CopyControl BAC clones of 110-145 kb increased >14-fold following addition of CopyControl Induction Solution. U=uninduced cells; I=induced cells.

 

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ-rpsL (StrR) nupG trfA dhfr

TransforMax EPI300 Electrocompetent E. coli

·         Transformation efficiency of >1 x 1010 cfu/µg of pUC19.

TransforMax EPI300 Chemically Competent E. coli

·         Transformation efficiency of >5 x 108 cfu/µg of pUC19.

*Covered by issued and/or pending patents.

 

Catalog No.

Concentration

Size


TransforMax™ EPI300™ Electrocompetent E. coli

EC300105

 

5 X 100 µl,

EC300110

 

10 X 100 µl

EC300150

 

50 X 100 µl

Includes CopyControl™ Induction Solution and pUC19 control DNA.


TransforMax™ EPI300™ Chemically Competent E. coli

C300C105

 

10 X 50 µl

Includes CopyControl™ Induction Solution and pUC19 control DNA.


CopyControl™ Induction Solution

CCIS125

 

25 ml

1,000X concentrated solution. Filter sterilized.

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